Email Us:  svkbioenterprises@gmail.com

Send Message

GUD GeneAmp BK Virus (BKV) Qualitative PCR Kit

  • Home
  • GUD GeneAmp BK Virus (BKV) Qualitative PCR Kit
BKV (BK Virus) Qualitative PCR Kit

GUD GeneAmp BKV (BK Virus) Qualitative PCR Kit:

PRODUCT CODE: Gene Amp-010

INTRODUCTION: BK Virus (BKV), a member of the polyomavirus family, is a common latent virus that can reactivate in immunocompromised individuals, particularly kidney transplant recipients. Reactivation can lead to BK virus-associated nephropathy (BKVAN), which may result in graft loss if not detected early.

The BK Virus PCR Detection Kit provides a fast, sensitive, and reliable method for the detection of BKV DNA in clinical samples using real-time PCR technology. Designed for diagnostic and research applications, this kit helps clinicians monitor viral Presence and guide timely medical intervention.

PRODUCT DESCRIPTION: GUD GeneAmp kit utilizes TaqMan probe-based real-time PCR to detect BK virus DNA with high specificity. Compatible with most real-time PCR systems, the kit includes all essential reagents for efficient workflow and accurate results.

❄️ Storage Conditions and Product Stability

  • All the kit reagents should be stored at -20 °C.
  • All kit components should be aliquoted based on your workload and stored at -20 °C after each use.
  • Avoid repeated thawing and freezing (more than 6–10 times) to prevent loss of assay sensitivity.

🧪 Kit Components

  • BKV Master Mix (Contains 10X PCR Buffer, 40 mM dNTP, 5U/µl Hot Start Taq DNA Polymerase)
  • BKV Primer Probe Mix (BKV PPM)
  • IC Primer Probe Mix (IC PPM)
  • BKV Positive Control (BKV PC)
  • MBGW (Molecular Biology Grade Water) – Negative Control

🧫 Sample Type

  • Blood
  • Urine

Note: Heparinized samples must not be used for isolation and further PCR analysis.

🧬 Assay Procedure

  1. BKV Sample DNA Extraction
  2. Preparation of Reaction Master Mix and Addition of Master Mix (Separately for BKV and IC)
  3. Preparation and Addition of BKV Primer Probe Mix (BKV PPM)
  4. Preparation and Addition of Internal Control Primer Probe Mix (IC PPM)
  5. PCR Programming
  6. Plate Setup (BKV – FAM, Internal Control – HEX)
  7. Result Interpretation (Separate analysis of BKV and Internal Control)

📦 Ordering Information

Product Code Description Reactions
GUD-BKV BKV PCR Kit 25 reactions
GUD-BKV BKV PCR Kit 50 reactions