PRODUCT CODE: Gene Amp-005
Simplify your cDNA synthesis workflow with our 2X RT Mix, a convenient,pre optimized master mix for fast and reliable reverse transcription. This all-in-one formulation contains reverse transcriptase RNase inhibitor, dNTPs, and optimized buffer in a single tube – just add your RNA and primer.
Applications:
Storage Temperature:
Store at -20°C
COMPONENT | VOLUME (µl) |
2x RT Mix (Reverse transcriptase RNase Inhibitor, dNTPs, Buffer) | 12.5 |
RNA Sample (1 µg) | 10.0 – 12.5 (Maximum) |
Water | Adjust based on RNA Concentration |
Total Volume | 25.0 |
Step | Temperature0C | Time | Cycles |
1 | 25 | 10 Min | 1 |
2 | 42 | 60 Min | 1 |
3 | 72 | 10 Min | 1 |
