PRODUCT CODE: GGA-004
RT Enzyme with high-efficiency unlocks superior cDNA. Engineered for optimal performance in RT-PCR, RT-qPCR, and cDNA library construction, this enzyme ensures robust and reliable transcription of RNA into complementary DNA (cDNA).
Applications:
Storage Temperature:
Store at -20°C
COMPONENT | VOLUME (µl) |
Random Hexamer’s | 5.0 |
RNase Inhibitor | 0.5 |
2x RT Mix (only Buffer and dNTP’s) | 12.5 |
RT Enzyme | 2.0 |
RNA Sample (1 µg) | 5.0 |
Total Volume | 25.0 |
Step | Temperature0C | Time | Cycles |
1 | 25 | 10 Min | 1 |
2 | 42 | 60 Min | 1 |
3 | 72 | 10 Min | 1 |
