PRODUCT CODE: Gene Amp-003
The 5X PCR Master Mix is a concentrated, ready-to-use mixture containing Taq DNA Polymerase, optimized reaction buffer, dNTPs, and MgCl₂. Designed to simplify routine PCR workflows, the mix reduces pipetting errors and ensures consistent performance across a wide range of DNA templates.
Applications:
Storage Temperature:
Store at -20°C
| Component | Volume (µl) |
|---|---|
| 5X PCR Master Mix | 10.0 |
| Forward Primer (10 pmol/µl) | 0.8 |
| Reverse Primer (10 pmol/µl) | 0.8 |
| Template DNA | 5.0 |
| Nuclease-free Water | 3.4 |
| Final Volume | 20.0 µl |
| Step | Temperature (°C) | Time | Cycles |
|---|---|---|---|
| 1 | 95 | 5 minutes | 1 |
| 2 | 95 | 30 seconds | |
| 52 | 45 seconds | 35 | |
| 72 | 1 minute | ||
| 3 | 72 | 5 minutes | 1 |
