GUD GeneAmp Hot start Taq DNA Polymerase

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GUD GeneAmp Hot Start Taq DNA Polymerase (5u/µl)

PRODUCT CODE:    GGA-002

Hot Start Taq DNA Polymerase is a thermo stable enzyme designed for high-specificity PCR by minimizing non-specific amplification and primer-dimer formation. The enzyme is inactive at room temperature and requires a heat activation step to initiate polymerase activity, enabling reliable and reproducible results in a wide range of PCR applications.

Applications: PCR and genotyping,High-throughput screening,Diagnostic assay development,Complex genomic DNA amplification

GUD GeneAmp Hot Start Taq DNA Polymerase can be used in the RTPCR Assays (Real Time PCR Assays), in the Master Mix Preparations.

    Unit Definition:

  • One unit is defined as the amount of enzyme that will incorporate 15nmol of dNTP into acid-
  • Insoluble material in 30 minutes at 75°C

     Storage Temperature: Store at -20°C

PCR Reaction Setup

ComponentVolume (µl)
5X Hot Start PCR Master Mix10.0
Pathogen Primer Probe Mix2.5
IC Primer Probe Mix1.5
Template DNA5.0
Nuclease-free Water1.0
Final Volume20.0 µl

PCR Cycling Conditions

StepTemperature (°C)TimeDye CapturingCycles
19410 minutes1 cycle
29415 seconds 
 5560 secondsYES40 cycles