We provide a comprehensive range of high-quality PCR enzymes and real-time PCR-based diagnostic kits under the GUD GeneAmp brand. Designed for sensitivity, specificity, and accuracy, these products cater to research labs, hospitals, and diagnostic centers for various genetic and infectious disease applications.
Here are some common questions and answers to help you better understand how to use and get the best results from our GUD GeneAmp PCR kits.
The shelf life of GUD GeneAmp PCR kits varies depending on the specific product, but typically they are stable for up to 12-24 months when stored correctly at the recommended temperature (usually -20°C). Always check the expiration date printed on the kit box for accuracy.
GUD GeneAmp PCR kits should be stored at -20°C for long-term storage. Once thawed, the kits should be kept at 4°C and used within a few weeks to maintain optimal performance. Avoid repeated freeze-thaw cycles as they can affect the product’s efficiency.
Yes, GUD GeneAmp PCR kits are designed to work with DNA extracted from a variety of sample types, including clinical samples like blood, tissue, and saliva. For best results, ensure that the DNA extraction method is compatible with the PCR amplification process.
The PCR conditions are typically optimized for general use; however, depending on the template or your experimental setup, slight adjustments to PCR conditions such as annealing temperature or cycle number may be necessary. We recommend following the guidelines provided in the product datasheet for the best results.
No, the GUD GeneAmp PCR kits come with all the necessary reagents required for PCR amplification (except for the template DNA or RNA). You’ll only need standard PCR equipment such as a thermal cycler and pipettes. Refer to the specific product manual for any additional setup requirements.
The GUD GeneAmp PCR kits are compatible with a wide range of sample types, including whole blood, plasma, tissue biopsies, saliva, and more. Specific sample preparation protocols are provided for each product to ensure optimal results. Always use fresh or properly stored samples for best accuracy.
GUD GeneAmp PCR kits are primarily designed for qualitative detection of specific targets such as pathogens, genes, or mutations. For quantitative applications, we recommend using a quantitative PCR kit or setup designed for measuring gene expression or viral load.
Common signs of PCR failure include no amplification products (no bands on the gel or no signal in real-time PCR), weak or inconsistent amplification, or primer-dimer formation. Troubleshooting can include adjusting template DNA quality, primer design, or PCR conditions, such as optimizing the annealing temperature or increasing the cycle number.
Inconsistent or weak results can be caused by several factors, including poor-quality DNA, incorrect template concentrations, improper handling, or expired reagents. Ensure that the DNA/RNA template is pure and intact, and follow the kit instructions carefully. If problems persist, our technical support team is available for troubleshooting
Many of our PCR kits, such as those for detecting HLA-B27 or HPV, are CE-IVD marked, meaning they are approved for use in diagnostic applications. However, please check the specific product's certification and intended use, as some may be restricted to research use only. Always ensure that you follow local regulatory requirements when using products for diagnostic purposes.
