Frequently Asked Questions (FAQs)One-Step RT-PCR combines reverse transcription and PCR in a single tube, saving time and reducing contamination risk. Two-Step RT-PCR separates cDNA synthesis and PCR into two steps, offering more flexibility for gene expression studies.
The kit includes a 2X One-Step RT-PCR Buffer and One-Step RT-PCR Enzyme Mix. It is optimized for efficient RNA to DNA amplification in a single reaction.
Yes, GUD RT-PCR kits are formulated with high-efficiency enzymes and buffers that ensure sensitive detection of low-copy RNA templates.
When stored at -20°C, all GUD kits are stable for at least 12 months from the date of manufacture.
Yes, GUD PCR and RT-PCR master mixes are compatible with standard thermal cyclers from major manufacturers like Bio-Rad, Thermo Fisher, and Eppendorf.
Hot Start Taq prevents non-specific amplification and primer-dimer formation by remaining inactive at room temperature. It becomes active only after the initial denaturation step.
Each kit is designed for 100 reactions of 20 µl each unless otherwise specified (e.g., the cDNA Synthesis Kit includes 50 reactions).
Yes, GUD GeneAmp 2X Master Mix and Hot Start Master Mix are suitable for multiplex PCR, but optimization of primer concentrations and cycling conditions may be necessary.
Gudgeneamp products are intended for research use only unless specified otherwise. For diagnostic use, please refer to our range of validated diagnostic PCR kits.
Store all enzyme components at -20°C. Avoid repeated freeze-thaw cycles. Thaw on ice and mix gently before use.
The kit contains RT enzyme mix, 5X reaction buffer, oligo dT primer, random primer, and dNTP mix—everything needed for efficient first-strand cDNA synthesis.
Yes, our technical team is available to assist with protocol development, troubleshooting, and optimization tailored to your specific application.
